Lokendra Kumar Sharma, Ph.D.
Research Interests: Mitochondrial Biology of Human Diseases, Oxidative stress, Mitochondrial diseases
Research Summary: Dr. Sharma’s research is focused on understanding the mitochondrial mechanisms of human diseases including cancer, aging and mitochondrial disorders. With an experience of more than 10 years in mitochondrial research at prestigious institutions in India and abroad, Dr. Sharma’s research contributed in understanding the critical role of mitochondrial alterations such as mtDNA mutations specifically in complex I subunits, that cause changes in oxidative status and oncogenic pathways and contribute in tumorigenesis and metastasis. His research also highlighted the rescue approaches for reversing the diseases phenotype through complex I recovery by genetic methods and mitochondrial targeted molecules in cellular and animal models of cancer. Similarly his recent research on maternally inherited mitochondrial diseases indicated that compromised mithagy is a major event in inducing mitochondrial defects and may contribute in developing clinical manifestations of these childhood diseases. Activating cellular recycling machinery and clearing damaged mitochondria in these cases could provide a viable approach to restore normal functions and to treat such devastating diseases. Given the fact that mitochondrial bioenergetics and metabolism are crucial for human health, changes in mitochondrial functionality could be used as reliable early predictive markers for disease progression and can also be used to target mitochondria for developing mitochondrial medicine for treatment regime.
Therefore current research is focused in the following areas:
Role of mitochondrial alterations and metabolism in carcinogenesis.
Understanding the mechanism of mitochondrial dysfunctions in maternally inherited mitochondrial diseases.
Development of mitochondrial based predictive markers for disease diagnostics (cancer, pregnancy complications and maternally inherited mitochondrial diseases).
Targeting mitochondria for developing therapeutic approaches.
For these investigations, cellular and animal models of human diseases are used for mitochondrial mechanistic studies. In addition, we have a strong collaborative network with clinicians/scientists in India and abroad for specific projects. Department is well equipped with state of art research infrastructure and funding support.
Sharma LK*, Tiwari M, Rai N, Bai Y*. Mitophagy activation rescues Leber's Hereditary Optic Neuropathy associated mitochondrial dysfunction and improves cell survival (Hum Mol Genet. 2019 Feb 1;28(3):422-433). (*Corresponding Author).
Edmunds LR, Sharma L, Wang H, Kang A, d'Souza S, Lu J, McLaughlin M, Dolezal JM, Gao X, Weintraub ST, Ding Y, Zeng X, Yates N, Prochownik EV. c-Myc and AMPK Control Cellular Energy Levels by Cooperatively Regulating Mitochondrial Structure and Function. PLoS One. 2015 Jul 31;10(7):e0134049. doi: 10.1371/journal.pone.0134049. eCollection 2015. (PMID:26230505) (eISSN: 0304-4165).
Mishur RJ, Khan M, Munkácsy E, Sharma L, Bokov A, Beam H, Radetskaya O, Borror M, Lane R, Bai Y, Rea SL. Mitochondrial metabolites extend lifespan. Aging Cell. 2016 Apr;15(2):336-48.] (PMID: 26729005) (ISSN: 1474-9726).
Lokendra Kumar Sharma*, Meenakshi Tiwari, Santosh Kumar Mishra. Mitochondrial Alteration: A Major Player in Carcinogenesis.Cell Biology Special Issue: Mitochondria: Implications in Human Health and Diseases. 2015; 3(2-1): 8-16. Published online February 10, 2015. (eISSN:2330-0183). (eISSN:2330-0183). (*Corresponding Author)
Sharma LK, Fang H, Liu J, Vartak R, Deng J, Bai Y. Mitochondrial respiratory complex I dysfunction promotes tumorigenesis through ROS alteration and AKT activation. Hum Mol Genet. 2011 Dec 1;20(23):4605-16. (PMID: 21890492). (e ISSN 1460-2083).
Dr. Lokendra Kr. Sharma
Department of Molecular Medicine & Biotechnology
4th Floor PMSSY Building
Sanjay Gandhi Post Graduate Institute of Medical Science (SGPGIMS)
Raebareli Rd, Lucknow, Uttar Pradesh 226014
Ph. 0522-2495384 (0)